Revvity, 61DB11RDF, HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL

This Low Ionic Strength Eu detection buffer is specifically designed for protein-protein interaction studies. It has been optimized for maximum performance when the studied protein-protein interaction involves a Europium cryptate conjugate in the assay and is sensitive to high ionic strength.
Evaluation of the two PPI Eu buffers in protein/protein interaction assays The two Europium PPI detection buffers were evaluated in two different Protein/Protein interaction assays. The IC50 values corresponding to the inhibition of the two interactions (A/B & C/D) remained comparable in both PPI Eu detection buffers. The Protein A/ Protein B interaction displayed the same Signal to Background (S/B) in the PPI Eu detection buffer and the PPI Low Ionic Strength Eu detection buffer. However, the S/B of the Protein C/ Protein D interaction was significantly improved in the new PPI Low Ionic Strength Eu detection buffer, suggesting that the PPI Low Ionic Strength Eu detection buffer can maximize assay performance when the studied protein-protein interaction is sensitive to high ionic strength.