Revvity, 62AMXADC, HTRF IBMX Phosphodiesterase Inhibitor, 500 µL

PDEs are represented by a large super-family of enzymes whose role is to hydrolyze cAMP into AMP, and cGMP into GMP. It should be emphasized that while not all PDEs lead to the specific hydrolysis of cAMP, the non-selective PDE inhibitor IBMX is often the preferred chemical used in most cAMP accumulation assays. The use of a Pan-PDE inhibitor ensures that the hydrolysis effect of most PDEs will be inhibited, which in turn guarantees high levels of cAMP accumulation in the cell. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays