Revvity, 62AMYADA, HTRF Forskolin Adenylyl Cyclase Activator, 40 µL

Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. It is used as a positive control for Gs applications ( biological models validation, maximal intracellular cAMP modulation determination). It is highly used for Gi coupled receptor study as a pre-activation step to reveal a cAMP inhibition upon cell stimulation. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays