Revvity, 63ADK056PEG, HTRF Human MANF Detection Kit, 500 Assay Points

Mesencephalic astrocyte-derived neurotrophic factor (MANF) (also recognized as ARMET or arginine-rich mutated in early stages of tumors) belongs to the fourth family of neurotrophic factors. MANF is an endoplasmic reticulum (ER) resident protein highly expressed in the developing cortex that plays an important role in protecting and repairing dopaminergic neurons in the rat 6-hydroxydopamine Parkinson disease model. MANF is also involved in protecting against various forms of endoplasmic reticulum stress. The human MANF kit enables the fast detection of MANF in cell supernatants and serum. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays
Assay principle Human MANF is measured using a sandwich immunoassay involving two specific human MANF antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of MANF present in the sample. Assay protocol The MANF assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 4-hour incubation period at RT.