Revvity, 64ALDPEG, HTRF Aldosterone Detection Kit, 500 Assay Points

The Aldosterone assay kit accurately measures the release of the Aldosterone steroid hormone in cell supernatant samples. Aldosterone is a steroid hormone synthesized from cholesterol in the adrenal cortex. Aldosterone is metabolized in the kidney and liver, and functions as the key mineralocorticoid in the control of sodium and potassium balance. The Aldosterone kit offers superior benefits over traditional ELISA readouts with the ease of an Add & Read process coupled with the robustness and sensitivity of homogeneous TR-FRET assays. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays
Assay principle The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and Aldosterone coupled to XL665 (acceptor). Aldosterone produced by cells competes with XL665-labelled Aldosterone for binding to monoclonal anti-Aldosterone Eu Cryptate. Assay protocol The Aldosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Aldosterone detection using HTRF reagents This protocol requires only a single, one-hour incubation period following cell stimulation.