Revvity, 64APPKAA, HTRF APP substrate Eu-Conjugate, 5,000 Assay Points

Beta-secretase (BACE1) is an enzyme which cleaves amyloid precursor protein (APP) to produce the amyloid ß42 peptide, responsible for insoluble amyloid plaques in Alzheimer's disease (AD). APP substrate, used with HTRF APP substrate labeled with both biotin and Eu3+ Cryptate, enables the study of High enzymatic Km to monitor beta-secretase (BACE1) activity. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays