Revvity, 65EU2ABB, HTRF Europium NH2 Labeling Reagent, 1 mg

Europium cryptate-NH2, is a do-it-yourself solution for labeling peptides, oligonucleotides or proteins with Europium cryptate.This reagent is ideal for experienced users familiar with labeling and chromatography. Used in combination with bi-functional reagents such as carbodiimides and SMCC/SPDP. Maleimide group generated by SMCC activation of the Europium cryptate-NH2 reacts with the thiol generated by SPDP/DTT treatment on the biomolecules to label. HTRF assays offer many advantages over other technologies: Homogeneous add-and-read format No wash steps Low background Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates Stable signal, providing flexibility in time of readout or size of assays
Assay protocol As shown here, preactivation of both the Europium cryptate and the biomolecule may be required. Recovery of labeled material can be achieved using conventional chromatographic methods, such as gel filtration or HPLC. The purification devices required for recovering labeled material are not delivered with these reagents.