Description
Glycan profiling and screening, super charged.
A high percentage of all biotherapeutics contain N-linked glycosylation. The impact of N-linked glycosylation on protein function can be significant. Thus, the characterization of N-glycan composition is critical for the biotherapeutic development process. The LabChip® Extended Range N-Linked Glycan assay provides a fast, efficient means to assess the main glycoforms of IgG and related proteins.
Glycoprotein Sizing, Glycoprotein Relative Quantification & Charge Variant N-Glycan Analysis
The LabChip® Glycan Profiling Assay Reagent Kit is ideal for rapid screening of N-linked glycan profiles by combining:
On-plate protein denaturation
Rapid enzymatic deglycosylation with PNGase F in 96 well format
Rapid electrokinetic separations and detection using LabChip® microfluidic chip technology
Per sample run time is under 45 seconds with a full 96 sample analysis in under 90 minutes
Automated peak detection and reporting of N-glycan relative abundance
Minimal sample consumption: Only 8 µL of sample required
Digital data format: Gel-like image, electropherogram, and tabular data formats with automated sample alignment, overlay, scaling and zooming features, allow users to conveniently share, store and archive data
The Glycan Profiling Assay Reagent Kit supports both neutral glycan and charged N-linked glycan found in MAbs. Drop down menus allow you to easily choose between assays.
To release and label glycoforms of IgG and related proteins, use the Glycan Release and Labelling Kit, before analyzing with the Glycan Profiling Assay Reagent Kit
