Revvity, AL3169C, AlphaLISA Human C1q Binding Kit, 500 Assay Points

Complement component 1q, also known as C1q, is a protein complex involved in the complement system. C1q senses antigen/antibody complexes formed with IgG or IgM and binds to antibody Fc via its globular heads. When this occurs, intramolecular rearrangement of the C1 complex is induced, triggering the activation of C1r and C1s proteases and the sequential cleavage of the circulating complement proteins. This leads to the formation of the Membrane Attack Complex (MAC) and to cell lysis. C1q binding measurements are related to the mechanism of action and Fc effector functions of biologics and therapeutic antibodies and utilized for studies in oncology, autoimmune disease, neurology, and infectious disease. Formats Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 20 µL reaction volume (5 µL of sample). Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 20 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 20 µL reaction volume (5 µL of sample). Features No-wash steps, no separation steps ELISA alternative technology Sensitive detection Small sample volume