Description
Formats:
Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Features:
No-wash steps, no separation steps
Alternative technology to a mouse IL-6 ELISA kit
Sensitive detection
Broad sample compatibility
Small sample volume
Results in less than 3 hours (Half the time of an IL-6 ELISA kit)
In the mouse, Interleukin 6 (IL-6) is a cytokine comprising 187 amino acids. In humans, IL-6 is produced by a variety of cell types, including T-cells, mast cells, and monocytes. IL-6 signals through binding to the gp130/ IL-6R receptor complex. IL-6 acts not only on the immune system, but also affects many physiological events in various organs. IL-6 exerts pro- or anti-inflammatory responses, depending on the target cell analyzed and the in vivo environmental circumstances. IL-6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver and acts as a survival factor on neuronal cells. Elevated serum IL-6 levels have been observed during bacterial and viral infection, trauma, autoimmune disease, inflammation, and cancer.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. The AlphaLISA Mouse Interleukin-6 (IL-6) Detection Kit is a superior alternative to a traditional mouse IL-6 ELISA kit.
Principle of the AlphaLISA assay
The AlphaLISA assay is based on an AlphaLISA sandwich immunoassay involving a biotinylated anti-analyte antibody bound to Streptavidin-coated AlphaLISA Donor beads and an anti-analyte antibody conjugated to AlphaLISA Acceptor beads. Both antibodies are directed against the analyte of interest. In the presence of the target, both antibodies bind to analyte and the beads come into proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer within the Acceptor beads, resulting in emission with λmax at 615 nm. The intensity of the signal is directly proportional to the concentration of analyte present in the sample.
Protocol of the AlphaLISA assay
The AlphaLISA assay can be run in a 96- or 384-well detection plate (50 µL final). As described here, samples or standards are dispensed directly into the assay plate for the detection of the analyte of interest by AlphaLISA reagents. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.
