Description
Overview
Tau aggregates are pathological assemblies of hyperphosphorylated tau protein, commonly observed in tauopathies such as Alzheimer's disease (AD), frontotemporal dementia (FTD), and progressive supranuclear palsy (PSP). Tau is a microtubule-associated protein that stabilizes axonal microtubules under normal conditions. In disease states, abnormal post-translational modifications such as hyperphosphorylation cause tau to detach from microtubules and misfold into toxic oligomers and fibrils, forming neurofibrillary tangles. These aggregates disrupt neuronal transport, synaptic integrity, and contribute to neuroinflammation and cell death. Therapeutic efforts aim to inhibit Tau aggregation, enhance its clearance, or block its spread between neurons.
The AlphaLISA
SureFire Ultra
Human Tau Aggregate Detection Kit is a sandwich immunoassay for the quantitative detection of Tau Aggregate in cellular lysates, using Alpha Technology.
Formats:
The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA
SureFire Ultra
kits are compatible with:
Cell and tissue lysates
Antibody modulators
Biotherapeutic antibodies
AlphaLISA
SureFire Ultra
kits can be used for:
Cellular kinase assays
Receptor activation studies
High-throughput screening for preclinical studies
How it works
Aggregation-AlphaLISA
SureFire Ultra
assay principle
The Aggregation-AlphaLISA
SureFire Ultra
assay measures the aggregates level of a protein target in a cell lysate.
The Aggregation-AlphaLISA
Surefire Ultra
assay uses one antibody which recognizes an exposed epitope on the aggregated target protein. AlphaLISA assays require two bead types: Acceptor and Donor beads. Acceptor beads are coated with a proprietary CaptSure™ agent to specifically immobilize the assay specific antibody, labeled with a CaptSure tag. Donor beads are coated with streptavidin to capture the detection antibody, which is biotinylated. In the presence of targeted protein, the antibody brings the Donor and Acceptor beads in close proximity whereby the singlet oxygen transfers energy to excite the Acceptor bead, enabling the generation of a luminescent Alpha signal. The amount of light emission is directly proportional to the quantity of aggregated protein present in the sample.
Aggregation-AlphaLISA
SureFire Ultra
two-plate assay protocol
The two-plate protocol involves culturing and treating the cells in a 96-well plate before lysis, then transferring lysates into a 384-well Optiplate plate before the addition of Aggregation-AlphaLISA
SureFire Ultra
detection reagents. This protocol enables the cells viability and confluence to be monitored. In addition, lysates from a single well can be used to measure multiple targets.
Aggregation-AlphaLISA
SureFire Ultra
one-plate assay protocol
Detection of aggregated target protein with AlphaLISA SureFire Ultra reagents can be performed in a single plate used for culturing, treatment, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust AlphaLISA SureFire Ultra quality.
