Revvity, ALSU-PIRF5-A-L, AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) Detection Kit, Control Lysate

Name: AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) Detection Kit, Control Lysate
Brand: Revvity
SKU: ALSU-PIRF5-A-L

Extra control lysate for the AlphaLISA ™ SureFire® Ultra™ Human Phospho-IRF5 (Ser446) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) assay kit, but can also be ordered separately. Extra control lysate for the AlphaLISA ™ SureFire® Ultra™ Human Phospho-IRF5 (Ser446) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) assay kit, but can also be ordered separately.

Catalog Number: ALSU-PIRF5-A-L

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Category

Oncology Molecular Diagnostic Kits

Pack Size1 vial

TagsImmunoassays Research & Development Protein Analysis Alpha Assays

Description

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-IRF5 (Ser446) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 300K/mL and incubated for 48 hours in T175 flasks in 10% FBS containing medium. Cells were harvested and resuspended at 8 x 106 cells/mL and then treated with 100 nM Calyculin A for 3 hours. Cells were then washed in HBSS + 0.1% BSA and lysed in Lysis Buffer at a final cell density of 8x106 cells/mL.

Specifications

General

Application	Cell Signaling
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Modality	Alpha
Lysis Buffer Compatibility	Lysis Buffer
Molecular Modification	Phosphorylation
Product Group	Control Lysate
Shipping Conditions	Shipped in Blue Ice
Target	IRF5
Target Class	Phosphoproteins
Target Species	Human
Technology	Alpha
Therapeutic Area	Inflammation Oncology
Unit Size	1 vial

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