Revvity, ALSU-PMLKL-B-L, AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate

Name: AlphaLISA SureFire Ultra Human Phospho-MLKL (Ser358) Detection Kit, Control Lysate
Brand: Revvity
SKU: ALSU-PMLKL-B-L

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-MLKL (Ser358) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) assay kit, but can also be ordered separately. Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-MLKL (Ser358) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) assay kit, but can also be ordered separately.

Catalog Number: ALSU-PMLKL-B-L

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Category

Protein Labeling Kits

Pack Size1 vial

TagsImmunoassays Research & Development Protein Analysis Alpha Assays

Description

This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-MLKL (Ser358) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in T175 flasks to 106 cells/mL in RPMI containing 10% FBS. Cells were treated with 20 μM Z-VAD for 30 minutes at 37°C, and then treated with 100 nM SM-164 and 20 ng/mL TNFα for 4 hours at 37°C. Total stimulus time 4.5 hours. Cells were then washed with HBSS and lysed in 5 mL 1X SureFire Ultra Lysis buffer.

Specifications

General

Application	Cell Signaling
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Modality	Alpha
Lysis Buffer Compatibility	Lysis Buffer
Molecular Modification	Phosphorylation
Product Group	Control Lysate
Shipping Conditions	Shipped in Blue Ice
Target	MLKL
Target Class	Phosphoproteins
Target Species	Human
Technology	Alpha
Unit Size	1 vial

Ordering

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