Revvity, AP178TB-R, AlphaPlex 545 (Terbium) Anti-6His Acceptor Beads, 25 mg

AlphaPlex®-545 Acceptor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture His-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for: Protein-protein interaction assays Protein detection assays Enzymatic assays AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay: 250 μg of Acceptor beads is sufficient to run 250-500 wells using a 50 μL reaction volume 5 mg of Acceptor beads is sufficient to run 5,000-10,000 wells using a 50 μL reaction volume 25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 μL reaction volume