Description
Alpha Donor beads conjugated to anti-FLAG antibody. The mouse monoclonal antibody M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites. These beads can be used to capture FLAG-tagged antibodies, proteins and peptides, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:
Analyte detection assays
Protein-protein interaction assays
Protein-DNA interaction assays
Protein-RNA interaction assays
Protein-small molecule interaction assays
Protein detection assays
Enzymatic assays
In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.