Revvity, AS116M, AlphaScreen Anti-6His Donor Beads, 5 mg

Alpha Donor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture 6XHis-tagged antibodies, proteins and peptides, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for: Analyte detection assays Protein-protein interaction assays Protein-DNA interaction assays Protein-RNA interaction assays Protein-small molecule interaction assays Protein detection assays Enzymatic assays In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.