Kit contains:
FFA120 Ladder (CLS158127, 1 vial, storage temperature -25 to -15°C)
FFA chip storage buffer (5 vials, storage temperature 2-8°C)
FFA marker (1 vial, storage temperature 2-8°C)
FFA gel matrix (6 vials, storage temperature 2-8°C)
Key Specification:
Sizing range: 20-120 nt
Concentration range: 4 nM – 2.5 µM Cy5-labeled DNA/RNA fragment
Sensitivity: 4 nM Cy5-labeled DNA/RNA fragment (before DMSO dilution)
Sizing resolution: 5 nt
Sizing reproducibility: CV < 5.0% or 4 nt
Run time: 60 seconds per well (~2.5 hours for 96 wells)
Chip preps for reagent kit: 5 HT preps or 10 LT preps
Maximum salt concentration: ≤ 20 mM Tris, 100mM KCl
Sample volume requirement: > 2 µL
Plates, Chips and Reagents sold separately:
HT DNA 5K/HT RNA LabChip® Assay (LabChip® GX Touch™ HT, 760435)
24 DNA 5K/HT RNA LabChip® Assay (LabChip® GX Touch™ 24, CLS138949)
Sipper wash solution (CLS158129)
Assay run program available from download:
FFA120. asyx LabChip® GX Touch™ Software v1.10 or higher and LabChip® GX Reviewer v5.8 or higher are required.
Citation:
Sun, Y. et al. (2023) ‘Application of microfluidic chip electrophoresis for high-throughput nucleic acid fluorescence fragment analysis assays’, NAR Genomics and Bioinformatics, 5(1). doi:10.1093/nargab/lqad011.
For more information contact:
[email protected]
