Description
PhenoVue Fluor 555 Live Cell Tubulin Stain is a cell permeable fluorogenic dye which specifically binds to polymerized tubulin and can be used for microtubule cytoskeleton imaging in live cells. PhenoVue Fluor 555 Live Cell Tubulin is a no-wash, sensitive, bright and photostable stain, which can be multiplexed with other blue, green, or far-red cell permeable stains such as PhenoVue Nuclear, Lysosomal or Mitochondrial stains.
Taxane derivatives, such as Paclitaxel and Docetaxel, which are commonly used in chemotherapy, inhibit microtubules depolymerization and cause cell cycle arrest. Like other tubulin stains derived from taxane, cytotoxicity and cell cycle impairment can be observed with long exposure time (>24h) at high concentration. This can be significantly limited at concentrations between 1 and 10 nM, while maintaining high brightness and image quality.
Depending on the cellular model, intracellular retention of PhenoVue Fluor 555 Live Cell Tubulin Stain can be further improved in the presence of efflux pump inhibitor, such as PhenoVue Probenecid, Ready to Use Solution.
Typical working concentration: 300 nM
Equivalent number of microplates:
6 to 20 x 96-well microplates
6 to 20 x 384-well microplates
10 to 32 x 1536-well microplates
