Description
Polyacrylamide gel electrophoresis (PAGE) offers superior size resolution for short nucleic acid fragments, making it the method of choice in workflows such as ribosome profiling.
PAGExt provides a streamlined, optimized workflow for extracting RNA directly from polyacrylamide gels, ensuring high recovery of size-selected material with minimal handling steps. The kit is specifically designed for RPFs purification, enabling clean isolation of fragments within defined size ranges for downstream library preparation.
PAGExt is also suited for final sequencing library clean-up, where gel-based size selection outperforms bead-based methods in resolving and removing primer dimers, adapter dimers, and other artifacts that can compromise sequencing quality and data interpretation.
All necessary buffers, filters, and consumables for gel extraction are included (polyacrylamide gels not included).
Key features
Rapid and efficient PAGE gel extraction of RNA fragments
Designed for RPF extraction and library size selection
Supports workflows requiring defined fragment ranges after PAGE separation
Suitable for ribosome profiling workflows that include an RPF gel extraction step
Streamlined extraction workflow aligned with downstream NGS library preparation needs
