Revvity, L0023GRE, Tag-lite Adrenergic β1 Receptor Green Antagonist Ligand

Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Beta1 adrenergic receptor green antagonist is a (+/-) Alprenolol derivative labeled with a green emitting HTRF fluorescent probe.
This ligand is best suited for use in the Adrenoceptor Beta1 receptor binding assay.
Tag-lite assays offer many features and applications over traditional receptor/ligand binding assays:
Non-radioactive
Homogeneous and filtration-free
Ready-to-use kits and reagents for binding assays
Does not alter the receptor pharmacology
Peer-reviewed, validated technology
Thorough kinetic studies with true K
d
and K
i
values, association (K
on
) and dissociation (K
off
) rate constants
Screening and profiling of biologics and large molecules
How it works
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.