Description
HIV p24 is the 230 amino acid phosphorylated protein of the capsid forming the conical core of the virus that encapsulates the HIV genomic complex. The Alliance HIV-1 p24 Antigen HIV ELISA assay is for the detection of HIV-1 and HIV-0 Subtypes in human serum, plasma and cell culture supernatants. In gene therapy, HIV-derived lentiviral vectors are useful and commonly used to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.
Here are some references demonstrating the p24 Alliance ELISA can be used for lentiviral transduction efficiency:
Valkama, A., Leinonen, H., Lipponen, E. et al. Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor. Gene Ther 25, 39–46 (2018).
Majdoul S, Cosette J, Seye AK, et al. Peptides derived from evolutionarily conserved domains in Beclin-1 and Beclin-2 enhance the entry of lentiviral vectors into human cells. J Biol Chem. 2017;292(45):18672–18681. doi:10.1074/jbc.M117.800813
Lesch, H., Laitinen, A., Peixoto, C. et al. Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Ther 18, 531–538 (2011).
Fenard D, Ingrao D, Seye A, et al. Vectofusin-1, a new viral entry enhancer, strongly promotes lentiviral transduction of human hematopoietic stem cells. Molecular therapy. Nucleic Acids. 2013
Ausubel LJ, Hall C, Sharma A, et al. Production of CGMP-Grade Lentiviral Vectors. Bioprocess Int. 2012;10(2):32–43.
This kit provides reagents for immune complex disruption (ICD) of antigen/antibody complexes in serum and plasma samples and proprietary antibodies increasing the sensitivity of the assay. This kit is intended for research purposes only and is not approved for diagnostic use.
