Revvity, NEV10020EX, IVISense Osteo 680 Fluorescent Probe (OsteoSense)

As opposed to other imaging modalities such as CT or x-ray which raise the risk of harmful irradiation to the subject, in vivo fluorescence imaging offers no radiation exposure concerns, and when performed with optimized hydroxyapatite-targeted probes such as IVISense Osteo, can image and quantify bone growth and elucidate the biology of bone remodeling. IVISense Osteo NIR fluorescent bisphosphonate imaging probes are designed to bind with high affinity to hydroxyapatite (HA) both in vivo and in vitro. Since HA is known to bind pyrophosphonates and phosphonates as well as synthetic bisphosphonates with high affinity, IVISense Osteo agents offer a safe and easy way to assess the subtle bone mineral changes that occur in bone growth, bone loss, and in diseases that damage bone, using subtherapeutic doses for imaging. IVISense Osteo 680 is excited by 668 ± 10 mn excitation light and emits at 687 ± 10 mn. Including IVISense Isteo 680 in careful experimental design allows for multiplexing with other channels and/or imaging times, and so can be paired with other probes to achieve maximum efficiency and flexibility in imaging.