Revvity, NOVA-4203-05S, NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

Straightforward workflow: two PCR steps with minimal cleanups; no custom sequencing primers required. Cleanup beads included: NEXTFLEX NGS Cleanup Beads are supplied with the kit, allowing PCR cleanups without purchasing separate beads. This reduces consumables cost and simplifies purchasing. Accurate taxonomic assignment: overlapping paired-end 2×300 reads across the V4 region on Illumina MiSeq and Element Aviti sequencers support robust error correction and ASV recovery. Flexible Input: validated conditions for 1–50 ng genomic DNA help maintain consistency across samples. Multiplexing Options: from 12 to 384 PCRII Barcodes are available to support projects of all sizes. Analysis: product variants with “S” in their SKU include Cosmos-Hub 16S analysis (DADA2 with SILVA) to generate quality-controlled ASV tables and interactive reports. Automation: protocols are available for Sciclone™ NGS, NGSx, and Sciclone G3 NGSx iQ™ Workstations to automate 16S sequencing. Use this kit when you want a dependable 16S V4 library prep that fits standard 2×300 sequencing on Illumina MiSeq or Element Aviti sequencers and hands off cleanly to downstream taxonomic analysis, including the Cosmos-Hub.