Revvity, NOVA-4204-04, NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep Kit

Straightforward workflow: two PCR steps with minimal cleanups; no custom sequencing primers required. Beads included: NEXTFLEX NGS Cleanup Beads come in the kit, so PCR cleanups are covered without a separate purchase, which can reduce consumables spend and simplify ordering. Confident taxonomy calls: overlapping paired-end 2×300 reads across the V3-V4 amplicon on Illumina MiSeq and Element Aviti enable strong error correction and ASV recovery. Flexible Input: validated conditions for 1 – 50 ng genomic DNA help maintain consistency across samples. Scalable indexing: choose from 48 to 96 PCR II barcodes (384 available upon request) to match project size. Built-in analysis option: SKUs with an “S” include Cosmos-Hub 16S analysis using DADA2 with SILVA, producing quality-controlled ASV tables and interactive reports. Automation ready: methods available for Sciclone NGS and NGSx workstations, including G3 NGSx iQ. Use this kit when you need reliable 16S V3-V4 library prep for standard 2×300 paired-end runs on Illumina MiSeq or Element Aviti, with a clean handoff to downstream analysis in Cosmos-Hub.