Revvity, NOVA-5132-32, NEXTFLEX Small RNA Sequencing Kit V4

The NEXTFLEX small RNA sequencing kit v4 uses patent-pending technology to provide a completely gel-free small RNA library preparation solution for Illumina and Element sequencing platforms. Completely gel-free protocol from purified miRNA or as little as 1 ng total RNA, no PAGE cleanup required Automation-ready on Sciclone™ G3 NGSx, Sciclone G3 NGSx iQ™, and Zephyr™ G3 NGS workstations for walk-away scalability Low-bias ligation plus dimer reduction chemistry improve data quality from challenging biofluids (plasma, serum, urine, CSF) and extracellular vesicles. Internal benchmarking shows up to a 45% increase in unique miRNA species detected from as little as 1 ng of total RNA, supporting robust differential-expression analysis and novel small RNA discovery Libraries ready in ~ 6 hours, supporting rapid biomarker and functional genomics studies Includes 384 Unique Dual Index (UDI) barcodes for high-throughput multiplexing and minimal index hopping on Illumina/Element platforms Quality assured through rigorous testing of every lot Explore our Dharmacon™ miRNA modulation reagents to enhance or inhibit miRNA levels as part of your functional studies.