Revvity, NOVA-5149-21, NEXTFLEX Rapid XP V2 DNA-Seq Kit

Single-tube enzymatic fragmentation, end-repair & A-tailing - DNA to sequencing-ready libraries in as little as 2.5 hours PCR-free option for ≥ 250 ng input plus a broad 100 pg - 1 µg range for low- and high-input samples with PCR amplification Built-in NEXTFLEX normalization beads - skip post-library quantitation and manual pooling Ultra-low adapter-dimer formation & uniform GC coverage for confident variant detection Up to 1,536 UDIs available for large, multiplexed studies 96 UDI-UMI barcode set enables molecular error-correction for rare variant calling Automation-ready workflow validated on Revvity Automation NGS workstations Quality assured through rigorous testing of every lot The NEXTFLEX Rapid XP V2 DNA-Seq Kit unites speed, data quality, and scalability: enzymatic fragmentation streamlines the workflow, proprietary normalization beads remove an entire QC step, and an optional PCR-free path preserves library complexity. Whether processing a handful of samples or a 96-well plate on a liquid-handler, you get consistent, unbiased libraries ready for Illumina and Element AVITI sequencing.