Revvity, NOVA-5188-02, NEXTFLEX Rapid DNA-Seq Kit 2.0

End-to-end WGS library-prep solution including reagents and magnetic beads for optional one- or two-sided size selection 1 ng – 1 µg genomic DNA input; PCR-free libraries possible at ≥ 250 ng ~ 3 h bench-to-sequencer workflow with < 45 min hands-on time Low GC bias (< 3 %) and low duplication, delivering uniform coverage across GC-rich or moderately degraded genomes Up to 1,536 unique dual-index (UDI) barcodes available; 96 UDI-UMI sets available for rare-variant detection Convenient 8, 48, or 96-reaction kit sizes keep cost per sample competitive Generates single- or paired-end libraries for Illumina and Element AVIT™ sequencing platforms Automation-validated on the Sciclone™ G3 NGSx and Zephyr™ G3 NGS systems Designed for mechanically fragmented DNA (e.g., Covaris®, Bioruptor®); no enzymatic fragmentation module needed High adapter-ligation efficiency ensures maximum library conversion, even at low DNA inputs.