Description
Overview
Ideal for analysis of samples containing complex mixtures of eukaryotic and bacterial rRNA
3 to 7-fold increase in coverage of SARS-CoV-2 and other genomes in nasopharyngeal (NSP) samples
Single workflow to detect viral genomic data, microbiome composition, co-infections, and host gene expression
Streamlined Jumpcode™ CRISPRclean workflow: stranded total RNA library prep with ribodepletion
Automated on the Sciclone™ G3 NGSx iQ™ liquid handler
Improve your discovery of high-value and biologically interesting transcripts
Powered by CRISPR-Cas9, ribodepletion is highly efficient and programmed to cut ribosomal RNA from human sequences (5S, 5.8S, 18S, 28S, 45S (precursor), mitochondrial 12S and 16S sequences), and 212 bacterial species from all phyla (5S, 16S, and 23S rRNA sequences).
Fully Automated Protocol
A tested method is available to automate Jumpcode Genomics’ CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit on the Sciclone™ G3 NGSx iQ™ liquid handler.
Multiplexing options
The NEXTFLEX™ RNA-seq 2.0 Unique Dual Index Barcodes (1-384) are provided at a concentration optimized for use with Jumpcode Genomics’ CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit.
