Description
Overview
Mitochondrial-derived fragments can account for a large share of reads in human ATAC-seq, DNA-seq, and bulk or single cell RNA-seq libraries, especially from mitochondria-rich tissues, reducing effective coverage of nuclear loci and low-abundance features. The NEXTFLEX Cas9-gRNA Mito Depletion Enzyme (Human) is a cost-effective, workflow-independent, and highly specific enzyme that reduces mitochondrial background, directing more sequencing capacity toward nuclear genes, regulatory elements, and other regions of interest, without requiring you to move to a new library prep kit.
It uses a DASH-style (Depletion of Abundant Sequences by Hybridization) CRISPR-Cas9 depletion approach, with a precomplexed Cas9 and pool of guides designed to cleave human mitochondrial DNA and selected nuclear mitochondrial pseudogenes at the double-stranded DNA stage. Whether you use your own prep method or a commercial kit, this module delivers clean, reproducible depletion of mitochondrial genome–derived sequences and improved coverage of nuclear content across diverse human NGS workflows.
Powered by Jumpcode™ DepleteX™ technology for specific mitochondrial sequence removal
Ready to use: provided as a Cas9-gRNA complex with integrated RNase inhibitor and 10X Cas9 Buffer
Fast: one-hour reaction time with only about 5 minutes of hands-on work
Convenient: easily fits into ATAC-seq, DNA-seq, RNA-seq, and single-cell workflows at the dsDNA stage
Flexible: compatible with short- and long-read sequencing
Automation-compatible: readily incorporated into manual or automated workflows
