Description
The PhenoVue autophagy staining kit provides a powerful and user-friendly solution for high-content imaging of key autophagy markers in fixed cells. It includes characterized, specific antibodies against:
LC3 (microtubule-associated proteins 1A/1B light chain 3B) which is involved in autophagosome membrane formation and elongation.
p62/SQSTM1, a selective cargo adaptor that accumulates when autophagy is impaired, serving as a readout for autophagic degradation.
LAMP1 (lysosome-associated membrane protein 1), a marker of lysosomes and autolysosomes involved in final cargo degradation.
The kit also includes PhenoVue Fluor secondary antibody conjugates, fixation/permeabilization buffers and a streamlined protocol designed for easy integration into high-content imaging workflows.
The kit is designed for optimal performance with Revvity’s PhenoPlate™ imaging microplates and has been verified on high-content screening systems like the Opera Phenix™ Plus, providing consistent results and experimental flexibility for advanced autophagy research.
Kit components /quantity
PhenoVue Hoechst 33342 Nuclear stain / 1 vial of 70 μL (500x)
PhenoVue anti-p62/SQSTM1 mouse IgG1 antibody Human only / 1 vial of 100 μL (100x)
PhenoVue anti-LAMP1 rat IgG2b antibody Human only / 1 vial of 100 μL (100x)
PhenoVue anti-LC3 rabbit IgG antibody Human, mouse and rat / 1 vial of 100 μL (100x)
PhenoVue Fluor 488 - Goat anti-mouse antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
PhenoVue Fluor 555 - Goat anti-rat antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
PhenoVue Fluor 647 - Goat anti-rabbit antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
PhenoVue Paraformaldehyde 4% solution / 1 vial of 25 mL (1x)
PhenoVue Permeabilization 0.5% Triton X-100 solution / 1 vial of 25 mL (5x)
PhenoVue Dye diluent A / 1 vial of 8 mL (5x)
