Description
PhenoVue neuronal differentiation staining kit includes four PhenoVue probes to visualize nuclei, actin, as well as B3 tubulin and nestin proteins.
This kit can be used to assess neuron differentiation efficiency from progenitors such as embryonic stem cells or induced pluripotent stem cells (iPSC), as well as to visualize neurons in brain cell derived co-cultures.
The combination of specific, bright and photostable PhenoVue dyes enables the ability to perform a 4-plex experiment with maximum spectral separation and a significantly reduced spectral overlap.
Each PhenoVue probe has been extensively validated and carefully optimized for excellent batch-to-batch reproducibility.
The PhenoVue neuronal differentiation staining kit offers a straightforward and user-friendly protocol to facilitate the experimental procedure.
Kit Components / Quantity
PhenoVue Hoechst 33342 nuclear stain / 1 vial of 70 µL (500x)
PhenoVue Fluor 555 - Phalloidin / 1 vial (600x)
PhenoVue anti-B3 tubulin antibody (mouse IgG2a) / 1 vial of 100 µL (100x)
PhenoVue anti-nestin antibody (mouse IgG1) / 1 vial of 100 µL (100x)
PhenoVue Fluor 488 - Rat anti-mouse IgG1 antibody highly cross-adsorbed / 1 vial of 200 µL (50x)
PhenoVue Fluor 647 - Rat anti-mouse IgG2a antibody highly cross-adsorbed / 1 vial of 200 µL (50x)
PhenoVue dye diluent A (5X) / 1 vial - 8 mL (5X)
PhenoVue paraformaldehyde, 4% solution / 1 bottle of 25mL
PhenoVue permeabilization 0.5% Triton X-100 solution / 1 bottle of 25 mL (5X)
