Revvity, RL00P-12, RiboLace Pro

RiboLace Pro enables selective enrichment of actively translating ribosomes for ribosome profiling (Ribo-seq) experiments. The technology uses a proprietary puromycin derivative (³P) coupled to magnetic beads to capture translation-engaged ribosomal complexes and the ribosome-protected fragments (RPFs) they contain following nuclease digestion. The antibody-free, tag-free enrichment workflow provides an alternative to gradient ultracentrifugation-based approaches and supports Ribo-seq sample preparation from primary or immortalized eukaryotic cell lines (fresh or frozen) as well as tissue samples. The recovered footprints are compatible with standard RPF library preparation workflows. Compatibility with the NEXTFLEX Small RNA-Seq Kit has been shown while remaining compatible with other common Ribo-seq library preparation approaches. Key features: Selective capture of actively translating ribosomes using ³P-functionalized magnetic beads Antibody-free, tag-free enrichment designed to avoid ultracentrifugation-based workflows Supports primary or immortalized eukaryotic cell lines (fresh or frozen) and tissue samples Enriches ribosome-protected fragments (RPFs) suitable for ribosome profiling and footprint sequencing Validated compatibility with the NEXTFLEX Small RNA-Seq Kit