Revvity, TE-BUF-KIT, AlphaLISA Lysis Buffer Selection Kit

This kit contains 10 mL each of buffers 1, 2, 3, 4, and 5 for use in cell-based target engagement assays. AlphaLISA TE Cell Lysis Buffer 1: This formulation is the most aggressive detergent formulation, containing divalent cation chelators, low salt concentration, moderate glycerol concentration. AlphaLISA TE Cell Lysis Buffer 2: Less aggressive detergent formulation, optimized for lysis of a broad range of cells without releasing nuclear DNA and minimally disrupting protein interactions; physiological salt concentrations. AlphaLISA TE Cell Lysis Buffer 3: Medium detergent concentration, strongest pH buffering capacity, no glycerol, low salt, 0.1% casein AlphaLISA TE Cell Lysis Buffer 4: Medium-High detergent concentration, contains divalent cation chelators, close to physiological osmotic strength, no glycerol AlphaLISA TE Cell Lysis Buffer 5: Medium-High detergent concentration, contains divalent cation chelator, close to physiological osmotic strength, highest glycerol concentration