Description
LANCE Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors. Features: Unmatched assay sensitivity and signal stability-even with overnight incubation. Optimized screening of difficult targets and Gαi antagonists. Easier detection of cAMP response of endogenous receptors-use fewer cells per well. Robust sensitivity when miniaturized to 1536-well format uHTS. Trusted results: stable pharmacology over time with consistent rank order potencies. Reproducible results with highest values LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
assay is based on competition between europium (Eu) chelate-labeled cAMP tracer and cellular cAMP binding sites on cAMP-specific monoclonal antibodies labeled with ULight dye. When antibodies are bound to Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites Eu chelate molecule of tracer. energy emitted by excited Eu chelate is transferred by FRET to ULight molecules on antibodies, which in turn emit light at 665 nm.
