Description
The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein (ANGPTL4) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Angiopoietin-like 4 (ANGPTL4), also known as HFARP, PGAR, or FIAF, is a 55 kDa glycoprotein that is structurally related to the angiopoietin family. It is secreted by the liver and fat tissue, and contains an N-terminal coiled-coil domain, and taC-terminal fibrinogen-like domain. Angiopoietin-like 4 plays a role in lipoprotein metabolism and angiogenesis. It is also involved in numerous cancers and is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and invasiveness.
