Revvity, TRF4016M, LANCE Ultra Total EGFR Detection Kit, 10,000 Assay Points

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4015S. Formats: Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume. Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume. LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000). The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase for a number of extracellular ligands in the EGF family. Ligand binding triggers a number of events including dimerization and auto-phosphorylation. Several major signaling cascades are activated by EGFR.