Sartorius, 18-5092, Octet® Amine Reactive 2nd Generation (AR2G) Biosensors

Name: Octet® Amine Reactive 2nd Generation (AR2G) Biosensors
Brand: Sartorius
SKU: 18-5092

The Octet® Amine Reactive 2nd Generation (AR2G) Biosensors are designed for EDC/Sulfo-NHS based immobilization of primary amine containing biomolecules and kinetic characterization of their interactions with target analytes. The use of the AR2G Biosensors requires the Amine Coupling Second-Generation Reagent Kit (AR2G Kit, Item no.: 18-5095). Octet® AR2G Biosensors are offered in a tray format. Each tray contains 96 biosensors. Larger orders include discounts. The 5-tray pack represents a 10% pe

Catalog Number: 18-5092

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Category

Target Enrichment Kits

Pack Size2 g

CollectionsSartorius Lab Filtration & Purification Solutions Flexsafe 2D & 3D Single‑Use Bags for Sterile Liquid Storage & Shipping Explore Sartorius TFF: Single‑Use & Scalable Crossflow Filtration

Description

The Octet®Amine Reactive 2nd-Generation (AR2G) Biosensors are coated with a newly engineered biocompatible surface matrix and are designed for kinetic characterization of interactions between purified proteins, peptides or other primary amines and target analytes. Immobilization of proteins is achieved through standard EDC-catalyzed amide bond formation to create a covalent bond between a reactive amine on the protein and the carboxy-terminated biosensor surface. The AR2G biosensors deliver enhanced performance and great signal intensity during both loading (ligand immobilization) and association (analyte detection) steps of an assay, while delivering reliable kinetic values.
The AR2G Biosensors’ surface chemistry makes it amenable to a wide range of pH and salt conditions, providing robustness and flexibility during the development of regeneration conditions for higher throughput applications.

Specifications

Applications

Application	Kinetics

Compliance Information

GMP Kits	No

Features

Device/Instrument Compatibility	Octet® R8; Octet® R4; Octet® R2; Octet® RH96; Octet® RED96e; Octet® RH16; Octet® N1; Octet® R8e

Product Information

Brand	Octet®BLI
Pack Size	1

FAQs

Question: What immobilization chemistry do Octet AR2G Biosensors use?

Answer:EDC/Sulfo-NHS activation couples primary amines on proteins or peptides to carboxyl groups on the sensor surface, forming covalent amide bonds.

Question: What additional reagent is required for AR2G assays?

Answer:The Octet Amine Coupling Second-Generation Reagent Kit is required for ligand immobilization and is purchased separately.

Question: How many biosensors are supplied per tray?

Answer:One tray contains 96 AR2G biosensors for biolayer interferometry kinetic assays.

Application Note

Octet® Amine Reactive 2nd Generation (AR2G) Biosensors provide a carboxyl-functionalized surface for covalent immobilization of proteins, peptides and other primary amine-bearing ligands. EDC/Sulfo-NHS activation enables amide-bond formation between the ligand and the biosensor surface, supporting biolayer interferometry (BLI) kinetic measurements without labeling the soluble analyte. The second-generation surface matrix accommodates assay optimization across different loading concentrations, pH values and buffer conditions. Each tray contains 96 biosensors for Octet BLI experiments. The Octet Amine Coupling Second-Generation Reagent Kit is required separately for surface activation and ligand attachment. These biosensors suit protein–protein interaction studies where a stable, covalently immobilized ligand is preferable to affinity capture.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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