Description
We combined our unique CIM®monolith technology with a standard multi-well plate design. Whether your goal is to screen multiple chromatographic conditions at once or high throughput purification of your target molecule, our CIM®plates offer an answer for you.
Each well in our monolithic plates is prefilled with a defined amount of monolith to enable uniform high flow rates across the plate. The properties of the monoliths in the wells are comparable to our preparative chromatographic monolithic columns. This makes plates an ideal tool for screening different parts of your chromatographic process.
CIM®plates can be disposable or multi-use and are made from medical grade polypropylene (PP), a material that prevents target molecules from binding to the plastic. They are manufactured according to ANSI standards and are automation compatible. Nevertheless, they can also be operated manually with a vacuum, centrifuge, or positive pressure.
PrimaS®HR features:
Multimodal chromatography ligand that combines elements of hydrogen bonding with weak anion exchange chromatography.
Selected to meet the need for highly reproducible purification processes (e.g., separation of full AAV capsids from empty AAV capsids)
Column release criteria based on the AAV8 elution profile.
AAV Binding at neutral or closer to neutral pH compared to QA-based columns: Multimodal properties of PrimaS®HR allow binding of AAV in neutral or closer to neutral pH values compared to QA-based columns.
Benefits
Simplicity of operation:No shaking and incubation required.
Easy transfer of the results to a larger scale:Monoliths in well-plates have the same properties as larger preparative monoliths (CIMmultus®), allowing simultaneous screening of multiple conditions and easy scale-up.
Time & money savings:Increased productivity by quick processing time and reduced sample consumption.
Monolith technology benefits:Up to 10-times greater capacities compared to other resin-based columns for large biomolecules due to high surface availability of binding sites. Low shears allow 3-times higher yields for particles and large biomolecules compared to other purification resins.
