Sartorius, BIA-814.5114-6, CIMmultus® DEAE 400 mL Monolithic Column (6 μm)

CIMmultus®Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production. CIMmultus®can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
CIMmultus®DEAE is a high-performance ion exchange column, primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.
DEAE features:
Weak anion exchanger
Working range, pH 2–9
Binds molecules with a predominantly negative charge, repels molecules with a predominantly positive charge
Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
Benefits:
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields