Answer:One vial contains 150 µg in 300 µL of 1 mM sodium citrate buffer at pH 6.4.
Answer:It is a 3,969-nt mRNA molecule with an approximately 95-nt poly(A) tail.
Answer:It is used for system suitability testing of chromatographic mRNA analytical methods, including PATfix workflows.
Sartorius mFix4 is an in-vitro-transcribed mRNA system-suitability standard comprising a 3,969-nucleotide RNA molecule with a polyadenylated tail of approximately 95 nucleotides. The material supports chromatographic evaluation of mRNA separations, including analytical system suitability on the PATfix mRNA platform. Each vial contains 150 µg of mRNA standard in 300 µL of 1 mM sodium citrate buffer at pH 6.4. The reference preparation contains 95 ± 2% polyadenylated mRNA. Store at −80 °C for long-term use, minimize repeated freeze–thaw cycles, and select analytical columns and conditions appropriate to the PATfix workflow. The standard is an analytical control, not an mRNA synthesis reagent.
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