Description
Plasmid pFix15 standard is a 15.4 kb plasmid DNA isolated from E. coli and chromatographically purified. It is mainly intended for System Suitability Testing and preparation of a standard curve. The standard contains 20% ± 2% OC and 80% ± 2% SC plasmid DNA isoforms.
Content:
1 x vial of 1 mL sample (250 µg of plasmid pFix15) (Cat. No. BIA-pFix15.1.1) - Packing buffer: 10 mM TRIS, 1 mM EDTA, pH 8.0
Equipment required:
PATfix®-model-pDNA (analytical system with quaternary pump) (Cat. No. PAT0029)
Consumables that are not included:
CIMac™ pDNA column (6 µm) (Cat. No. 150.8501-6), vials for the PATfix™ system
0.22 µm PES filter
Standard storage:
Long term storage: -80°C
Short term storage: pFix15 can be stored up to 6 months at -20°C
Standard shipment at 2-8°C. Standard is stable up to two weeks at this temperature. Avoid multiple freeze-thaw cycles.
