| Instruments | PA 800 Plus |
|---|
Answer:Approximately 10 to 225 kDa for SDS-MW protein sizing.
Answer:Three 100 µL portions at 16 mg/mL.
Answer:No. It supplies protein size references for a compatible SDS-MW analysis workflow.
The SCIEX Molecular Weight Sizing Standard is a protein reference mixture used to establish apparent molecular-weight calibration in SDS-based capillary electrophoresis. The standard spans approximately 10–225 kDa, supporting the assignment of migration-time-based molecular sizes to protein bands and peaks in relevant SDS-MW methods. The 3-pack configuration contains three 100 µL reference portions at 16 mg/mL. During an SDS-MW workflow, the protein mixture is prepared and separated under the conditions specified for the instrument and assay, allowing its components to serve as molecular-mass reference points. A consistent sizing standard is especially useful when evaluating antibody fragments, intact protein species and related purity or heterogeneity profiles. Treat apparent molecular weight as method-dependent: SDS binding, protein conformation and separation chemistry may cause departures from calculated sequence mass. Avoid changes to sample heating, reducing conditions and injection settings between standard and unknown samples without method verification. This is a sizing reference mixture, not a complete SDS-MW reagent kit or a capillary cartridge.
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