Fast and practical high resolution separation and quantitation of charged and polar analytes.
The P/ACE series is an automated, programmable capillary electrophoresis system designed to perform fast separations from complex samples. Separations occur in a capillary which is housed in an interchangeable cartridge with circulating liquid coolant for efficient temperature control. This finely regulated temperature control allows the use of high ionic strength buffers and large-bore capillaries necessary for analyzing difficult samples. With CE, methods are faster to develop, easier to validate, and less expensive to run. Note that different detectors are available to optimize performance for your application.
For every researcher there is an ideal analytical technology—one that helps you achieve your goals.
P/ACE MDQ Plus Systems Overview
P/ACE MDQ Plus system with UV detector - B52521 (optional PDA and LIF detectors)
The system includes a selectable-wavelength high sensitivity modular UV/Vis detector (includes 200, 214, 230 and 254 nm filters to optimize analyte specificity), UV source optics, temperature-controlled sample storage module and 32 Karat™ Software configured on a desktop controller. Installation Qualification, Operation Qualification 1 (OQ1) and documentation to aid in software validation is also included.
P/ACE MDQ Plus system with PDA detector - B52521PDA (optional UV and LIF detectors)
Features
Built-in auto-sampler and a programmable array of 36 pairs of buffers
Exclusive circulating liquid, precisely regulates capillary temperature
Sample temperature control
Integrated interchangeable detector modules
Methods development with 36 position buffer array
CAESAR integration algorithms
Automatic standards co-injection
Multi-mode electrokinetic, pressure and vacuum sample introduction
Internal fluidic system with variable pressure or vacuum
Robust sample interface
Sample from vials, micro vials or 96-well plates
Fully programmable separation methods
Easy to maintain
Applications
Basic drugs
Chiral
SSCP analysis
Quantitation by direct hybridization
A robust sample injection design for demanding analytical requirements
Automated sample handling
The P/ACE MDQ Plus system offers fully automated methods and extended sample-handling capability for walkaway operation.
Sampling can be performed using 1.8 ml universal vials, 96-well plates and micro vials.
Precision-molded polymethylpentene universal vials accommodate run buffer, sample and micro vials.
Quick-change electrodes
System electrodes and vial cap openers simplify user interaction, are designed to assure high strength and rigidity and are easily removed for maintenance.
Universal vial and cap design
Prevents the capillary and electrode from physically interacting with the caps, creating a better seal, and ultimately allowing for a robust, clean sample interface.
Sample interface
Provides a robust pressure seal ensuring accurate and reproducible data.
Built-in auto-sampler, pressure and vacuum, and sample and temperature control
Capillary temperature control
Efficient CE separations rely on precise capillary temperature regulation to manage Joule heating within the capillary. Proper temperature control plays important role in the repeatability of analysis.
Recirculating liquid coolant provides effective heat dissipation when performing assays
Capillary temperature can be regulated between 15 - 60°C.
Capillaries housed in cartridges facilitate temperature control and easy exchange of capillary dimensions and surfaces
Sample temperature control
Settable temperature between 4 - 60°C helps maintain sample stability or perform functional assays.
Multiple modes of sample introduction and separation
The P/ACE MDQ Plus system provides electrokinetic, pressure and vacuum introduction of samples. Introduction from either end of the capillary allows both ultrafast and high-resolution analyses.
Separations can be adjusted by varying voltage, current, pressure and vacuum
Combination of voltage and pressure ensures the buffer stays free of air bubbles which can be generated from outgassing
Variable pressure and vacuum
The internal fluidic system operates with all common rinsing protocols, regulating these with a pressure-handling capability of -5 to 100 PSI
Capillary conditioning is accomplished by moving specific volumes of electrolytes, gels, regenerants and cleaning solutions through capillary
Gel buffers are quickly and efficiently pumped into capillary
Our capillary electrophoresis (CE) technology is used in numerous applications including academic research, industry, small molecule drug development and quality control.
Forensics / Drug screening
While the highly polar nature of basic pharmaceuticals makes Liquid Chromatography complex, CE has the ability to excel at such separations. The robust nature of this tool and the ruggedness of this particular approach are appropriate for screening protocols used in forensic toxicology.
Food and beverage manufacturing
The MDQ Plus system, provides an in-depth analysis of food compounds including anions, cations, organic acid content, and protein integrity, to better manage product process consistency, taste, quality, storage conditions, safety, adulteration and authentication.
Petrochemicals
Analysis of anion and cation content gives you a better understanding of the chemical makeup of petroleum and related products. For example highly purified terephthalic acid (PTA) and its esters are major components in the production of plastics and fibers like polyethylene glycol and polyester. The purity of PTA has a direct effect on the quality and color of the end products.
Biofuels
The P/ACE MDQ system plus already excels in ion analysis and is a natural fit for biofuel production. The fermentation of lignocellulosic biomass to ethanol[1] is an attractive route to fuels that supplements fossil fuels. Incorporating, ion analysis, protein purity assays, isoelectric focusing and sugar analysis by capillary electrophoresis is invaluable in monitoring the fermentation and purification processes used in ethanol production.
Water quality and environmental
With ever-increasing water demands, quick and efficient identification of impurities, analytes, or other contaminating molecules within a water sample is a key step in managing the safety of our water supply. Water is also a vital component in the industrial production of semiconductors, metal plating, and is used in large volumes in nuclear power plants and pulp and per mills. The P/ACE MDQ Plus system identifies analytes that lead to corrosion as well as contaminants that contribute to downstream environmental pollution.
Specifications
System specifications
Dimensions:
Height: 29.2 in (74.2 cm)
Door open: 38.8 in (98.6 cm)
Width: 25 in (63.5 cm)
Depth: 28.4 in (72.1 cm)
Weight (uncrated):
188 lbs (85.3 kg) (includes UV/Vis detector)
Electrical requirements:
Voltage: 100 - 240 V; 50/60 Hz
Voltage range: 1 to 30 kV programmable at 0.1 kV increments
Current range:
3 to 300 μA programmable at 0.1 μA increments
Pressure delivery range:
-5 to +100 PSI
Sample temperature control:
4 - 60°C
Capillary temperature control:
15 - 60°C
System capacity
Sample trays:
2 x 96-well plates
2 x 48 universal vials
2 x 48 0.3 ml vials or microfuge tubes
Buffer tray:
2 x 36 universal vials
Detection capability
UV (optional)
200, 214, 254, 280 nm standard filter
190 - 600 nm (custom filter option)
Diode array (optional)
190 - 600 nm programmable
0.5 - 32 Hz scan collection frequency programmable
Laser induced fluorescence (LIF) (optional)
300 - 700 nm excitation range
350 - 750 nm emission range
0 - 1000 RFU
Source lasers with 3 mW power output:
488 nm solid-state laser (included in A66528)
635 nm diode laser (optional)
Supplies and accessories
Universal vial caps (100 pack): P/N A62250
Universal vials (100 pack): P/N A62251
200 μL microvials (100 pack): P/N 144709
NanoVials (100 pack): P/N 5043467
Electrode assembly: P/N A47775
Vial cap opener: P/N A95348
Vial holder assembly, 6x6 (36 vials): P/N A94462
Vial holder assembly, 6x8 (48 vials): P/N A94461
Capillary cartridge, semi assembled (no capillary): P/N A11147
Blank cartridge assembly kit: P/N 144738
Cartridge rebuilding kit: P/N 144645
Cartridge tubing: P/N 144689
Capillary cartridge coolant (450 mL): P/N 359976
