本文建立了依托泊苷有关物质分析的HPLC测定方法。参照《中国药典》中色谱分析条件,采用色谱柱Shim-pack VP-Phenyl和Shim-pack Scepter Phenyl-120,对依托泊苷有关物质系统适用性溶液、供试品溶液、对照溶液、灵敏度溶液进行分析,系统适用性溶液色谱图中,依托泊苷峰的保留时间约为26.593和26.307分钟,依托泊苷峰与对羟基苯甲酸丙酯峰的分离度符合要求,满足《中国药典》需求,此方法可为依托泊苷有关物质分析提供参考。
| 型号 | 反相/正相/HILIC色谱柱 |
|---|---|
| 货号 | 228-59928-92-SC |
| 类型 | 色谱柱 |
| 规格1 | 5um |
| 规格2 | 4.6x250mm |
| 变体ID | 61231 |
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