Takara, 3265, pRI 201-ON DNA

Use pRI 201-AN DNA to transform dicotyledonous plants. Use pRI 201-ON DNA to transform monocotyledonous plants. The presence of 2 multicloning sites enable multigene transformation with a single vector. Vectors contain a replication origin (ColE1 ori) derived from pUC plasmids, which allows maintenance at a high-copy- number inE. coli.pRI 201 DNA series are binary vectors designed for expressing target genes in transformed plant cells. This series of vectors retain the backbone of pRI 101 vectors (Cat.# 3262/3263), which includes an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5' -UTR) (translational enhancer region) downstream of the 35S promoter from cauliflower mosaic virus (CaMV). In addition, they have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 series 2. Further, multigene transformation with a single vector is possible by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the cloning site (MCS2) downstream of the HSP terminator.The pRI 201 series has two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. Carrying anArabidopsisADH-derived 5' -UTR (AtADH 5' -UTR), pRI 201-AN DNA is suitable for dicotyledonous plants. With a rice ADH-derived 5' -UTR (OsADH 5' -UTR), pRI 201-ON DNA is compatible with monocotyledonous plants.