Takara, 3365, pCold™ TF DNA

The pCold TF DNA Vector provides cold shock technology for high-yield protein expression combined with Trigger Factor (chaperone) expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins. pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) that facilitates co-translational folding of newly expressed polypeptides. Because of itsE. coliorigin, TF is highly expressed inE. coliexpression systems.The pCold TF DNA Vector consists of thecspApromoter plus additional downstream sequences, including a 5' untranslated region (5' UTR), a translation enhancing element (TEE), a His-Tag sequence, and a multiple cloning site (MCS).Alacoperator is inserted downstream of thecspApromoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between TF-Tag and the Multiple Cloning Site (MCS) and facilitate tag removal from the expressed fusion protein.E. colistrains can serve as expression hosts.