Takara, 3372, pCold™ GST DNA

The expression vector pCold GST DNA was developed by incorporating glutathione S-transferase (GST) derived fromSchistosoma japonicumas a soluble tag. The proteins expressed using this vector have an N-terminal GST tag, which can improve the stability and solubility of the fused protein. The pCold GST vector includes a 5' untranslated region (5' UTR), translation enhancing element (TEE), his tag, GST tag, and multiple cloning site (MCS) downstream of thecspApromoter. In addition, alacoperator has been inserted downstream of the promoter to allow precise control of gene expression. Finally, because the pCold vector series uses anE. colipromoter, virtually any strain ofE. colican be used as a host for protein expression.