Takara, 3380, B. Subtilis Secretory Protein Expression System

Bacillus subtilishas become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed inBacillusspecies such asB. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, theB. subtilisSecretory Protein Expression System from Takara Bio allows rapid development of a library ofB. subtilisclones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.