Description
pDON-AI-2 DNA enables production of a retrovirus vector with high transfer efficiency. By transfecting the vector into an appropriate packaging cell line, the vector expresses transient or stable transcribed product containing virus-packaging signal (Ψ) and target gene. This vector possesses LTR and Ψ (viral packaging signal), but not the structural gene necessary for particle formation and replication, which aregag,pol, andenv, and contains HCMV IE promoter within the U3 region of 5'LTR. pDON-AI-2 Neo DNA contains a neomycin resistant gene as a selective marker. The production of 2–8-fold higher-titer virus can be achieved by transfecting a version of pDON-AI-2 DNA containing a target gene compared using conventional retrovirus vector.
