Takara, 3658, pDON-5 DNA

This product is a plasmid vector for producing a high expression efficiency retrovirus vector. By transfecting this vector into an appropriate packaging cell line, the vector transiently or stably expresses transcribed products containing the virus-packaging signal (Ψ) and target gene. This vector possesses LTR and Ψ (viral packaging signal), but not the structural genes necessary for particle formation and replication (sequencesgag,pol, andenv). Because this vector contains an intron with high splicing capacity, it yields high transcription efficiency. This vector contains the HCMV IE promoter (which can produce high-titer retroviruses) within the U3 region of the 5' LTR, and a neomycin resistant gene as a selective marker.